Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94).doc

Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94)

在台灣地區的華人男性和女性志願者的血漿中之 [穀胱甘肽 （glutathione）] 的濃度（水平），隨著年齡的增長而逐漸地下降。

// - - Begin memo item - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - //
(Memo Item created on February 20, 2009 02:33 PM)
- - - Begin title or keyword:
Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94)

http://highwire.stanford.edu/cgi/medline/pmid;8749248?maxtoshow=&HITS=&hits=&RESULTFORMAT=&fulltext=Effect+of+ageing+on+human+plasma+glutathione+concentrations&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT
- - - End title or keyword:

Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection.
CS Yang, ST Chou, L Liu, PJ Tsai, and JS Kuo
J Chromatogr B Biomed Appl, December 1, 1995; 674(1): 23-30.

Department of Medical Research. Taichung Veterans General Hospital Taiwan (台中榮民總醫院) .

A convenient method for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves direct addition of human plasma to methanolic monobromobimane, for simultaneous protein precipitation and thiol derivatization. The assay was validated by addition of authentic GSH and GSSG to plasma samples. Plasma glutathione levels in Chinese male and female volunteers were found to decrease with increasing age (age groups 20-30, 30-40, 40-50, 50-60, and > 60; mean +/- S.E.M. 0.95 +/- 0.03, 0.77 +/- 0.02, 0.67 +/- 0.03, 0.51 +/- 0.02, 0.48 +/- 0.02 microM for male volunteers and 1.11 +/- 0.06, 0.76 +/- 0.03, 0.61 +/- 0.03, 0.53 +/- 0.04 and 0.43 +/- 0.04 microM for female volunteers). GSSG levels, in both males and females, did not show a correlation with age. There were no significant differences in GSH or GSSG levels among male and female volunteers of the same age group. These results suggest that elderly persons might be more susceptible to oxidative injury due to decreased plasma glutathione levels.

Publication Types:
Journal article

PMID: 8749248

--------------------------------------------------------------------------------
MEDLINE data is licensed by HighWire Press from the National Library of Medicine. Some material in the NLM databases is from copyrighted publications of the respective copyright claimants. Users of the NLM databases are solely responsible for compliance with any copyright restrictions and are referred to the publication data appearing in the bibliographic citations, as well as to the copyright notices appearing in the original publications, all of which are hereby incorporated by reference.
// - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - End memo item - - //